Journal: Biomarker Research

Article Title: Identification of GRIN2D as a novel therapeutic target in pancreatic ductal adenocarcinoma

doi: 10.1186/s40364-023-00514-4

Figure Lengend Snippet: Memantine is a potential drug for PDAC therapy. ( A ). Dose-response curve of memantine in PDAC cells. ( B ). Cell growth was inhibited in memantine treated PDAC cells, as revealed by CCk8 cell viability assay. ( C ). Cell migration was inhibited in memantine treated PDAC cells, as revealed by wound healing cell migration assay. ( D ). Clonogenic ability was inhibited in memantine treated PDAC cells, the cells in the colony formation assay were stained by crystal violet. ( E ). Level of GRIN2D decreased in memantine treated PDAC cells. ( F ). p38, MSK, and CREB in p38 MAPK signaling pathway were dephosphorylated after treatment of memantine. Data are from at least three independent experiments. Mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: Memantine (MedChemExpress, HY-B0365A).

Techniques: Viability Assay, Migration, Cell Migration Assay, Colony Assay, Staining

Journal: Journal of neurochemistry

Article Title: Long-lasting effect of NMDA receptor antagonist memantine on ethanol-cue association and relapse.

doi: 10.1111/jnc.13350

Figure Lengend Snippet: Fig. 1 Conditioning of lever-pressing behaviour in (a) animals that received a 5-min memory reactivation session followed by either vehicle or memantine treatment (n = 17/17) and (b) animals that were not subjected to the memory reactivation session (n = 8/8). Data are shown as the average number of lever presses on the ethanol- associated (active) and on the inactive lever. Data are presented as means SEM.

Article Snippet: One group received an intraperitoneal injection of vehicle (isotonic saline) and another group received 20 mg/kg of memantine (LKT Laboratories, St. Paul, MN, USA).

Techniques:

Journal: Journal of neurochemistry

Article Title: Long-lasting effect of NMDA receptor antagonist memantine on ethanol-cue association and relapse.

doi: 10.1111/jnc.13350

Figure Lengend Snippet: Fig. 2 Effect of either vehicle (n = 17) or 20 mg/kg of memantine (n = 17) on cue-induced ethanol-seeking behaviour. This test was performed 24 h after a 5-min memory reactivation session (a). The drug administration was performed twice, immediately and 4 h, after the reactivation session. Two additional experimental groups (n = 8/8) received the same treatment except the memory reactivation session (b). Data are shown as the average number of lever presses on the ethanol-associated (active) lever and on the inactive lever. Responses on the active lever were followed by the presentation of ethanol discriminative stimuli. Data are presented as means SEM. *indi- cates significant differences from the vehicle control group, p < 0.05.

Article Snippet: One group received an intraperitoneal injection of vehicle (isotonic saline) and another group received 20 mg/kg of memantine (LKT Laboratories, St. Paul, MN, USA).

Techniques: Control

Journal: Journal of neurochemistry

Article Title: Long-lasting effect of NMDA receptor antagonist memantine on ethanol-cue association and relapse.

doi: 10.1111/jnc.13350

Figure Lengend Snippet: Fig. 3 Extinction of lever-pressing behaviour in (a) animals that received a 5-min memory reactivation session followed by either vehicle or memantine treatment (n = 17/17) and (b) animals that were not subjected to the memory reactivation session (n = 8/8). Extinction sessions started 1 week after the memory reactivation session in drug- free animals. Data are shown as the average number of lever presses on the ethanol-associated (active) and on the inactive lever. Responses on both levers had no programmed consequences. Data are presented as means SEM.

Article Snippet: One group received an intraperitoneal injection of vehicle (isotonic saline) and another group received 20 mg/kg of memantine (LKT Laboratories, St. Paul, MN, USA).

Techniques:

Journal: Journal of neurochemistry

Article Title: Long-lasting effect of NMDA receptor antagonist memantine on ethanol-cue association and relapse.

doi: 10.1111/jnc.13350

Figure Lengend Snippet: Fig. 4 Reacquisition of lever-pressing behaviour in (a) animals that received a 5-min memory reactivation session followed by either vehicle or memantine treatment (n = 17/17) and (b) animals without the memory reactivation session (n = 8/8). Reacquisition testing was performed 24 h after the last extinction session in drug-free animals. Note: Groups with (a) and without (b) reactivation were run in two independent experiments. This explains slight differences in operant responding in vehicle-treated animals. Data are shown as the average number of lever presses on the ethanol-associated (active) lever and on the inactive lever. Responses on the active lever were followed by the presentation of ethanol discriminative stimuli and the delivery of 30 lL of 10% ethanol. Data are presented as means SEM. *indicates significant differences from the vehicle control group, p < 0.05.

Article Snippet: One group received an intraperitoneal injection of vehicle (isotonic saline) and another group received 20 mg/kg of memantine (LKT Laboratories, St. Paul, MN, USA).

Techniques: Control